Product name:Cellulase
CAS:9025-56-3
Specification:150ku
Description:
Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze the cellulolysis (or hydrolysis) of cellulose. However, there are also cellulases produced by other types of organisms such as plants and animals. Several different kinds of cellulases are known, which differ structurally and mechanistically. The EC number for this group of enzymes is EC 3.2.1.4.
Cellulase is a group of enzymes that catalyze the degradation of cellulose hydrolysis to produce glucose and fiber with low degree of polymerization, including three major components, glucan endonuclease, glucan exonuclease and fibrodisaccharase. It is not a single enzyme, but a multicomponent enzyme system that plays a synergistic role. Cellulase is a grayish white powder or liquid. In 1906 Seilliere discovered cellulase in the digestive juices of snails. Cellulase hydrolyzes the beta-1, 4-glucoside bond in the fiber to produce a soluble polymer and d-glucose. Depending on the source of the enzyme, its molecular weight ranges from 5,000 to 400,000. The optimal pH value is 4.0 ~ 5.0. The optimum temperature is 40 ~ 60℃. Some metal ions Mg2+, Cl2+ and neutral salts can activate this enzyme, while some other metal ions Ag2+, Cu2+, Mn2+, Hg2+ and dyes can deactivate this enzyme.
Cellulase has been widely used in various fields such as textile, daily chemical industry, paper making, food fermentation, industrial washing, tobacco, petroleum exploitation, waste water treatment and feed. Natural cellulase is found in microorganisms, some insects and plants. When seeds germinate, cellulase hydrolyzes seed coat cellulose, which is beneficial to seedling emergence. The symbiotic bacteria and protozoa in the digestive tract of herbivorous ruminants secrete cellulase, which hydrolyzes cellulose into sugars that can be absorbed. High quality feed and glucose can be produced by using cellulase secreted by microorganism. The application of trichoderma green (a kind of fungus rich in cellulase) to feed fermentation in agriculture can improve the nutritional value of cellulose feed. Cellulase products are one of the main reagents for the separation of plant protoplasts. In plant cell fusion experiments, cellulase or snail enzyme is commonly used to decompose and remove cell walls in order to separate the protoplasts needed.