Product name:Rat Tissue Polypeptide Antigen,TPA ELISA Kit
Description:
Intended use
This immunoassay kit allows for the specific measurement of rat Tissue polypeptide antigen (TPA) concentrations in serum and plasma.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPA present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for TPA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPA bound in the initial step. The color development is stopped and the intensity of the color is measured.