Product name:Rat Interleukin 1,IL-1 ELISA Kit
Application:ELISA
Quantity:96T
Species:Rat
Restrictions:For Research Use only.
Descripton:
Intended use:This immunoassay kit allows for the specific measurement of total rat IL-1 concentrations in cell culture supernates, serum, and plasma.
Introduction:. Cells known to express IL-1 include osteoblasts, macrophages, fibroblasts, keratinocytes and glial cells. The diverse functions attributed to IL-1 include T cell activation with subsequent IL-2 secretion and IL-2 receptor expression, increased synthesis of acute phase proteins by hepatocytes, and regulation of bone metabolism in developing bone.
Test principle:This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IL-1 is added to the wells.The color development is stopped and the intensity of the color is measured.