Product name:Rat endotoxin,ET ELISA Kit
Method:ELISA
Quantity:96T
Species:Rat
Application:For Research Use only.
Intended use:
This immunoassay kit allows for the in vitro quantitative determination of rat ET concentrations in serum, plasma and other biological fluids.
Introduction:
Endotoxins are potentially toxic, natural compounds found inside pathogens such as bacteria.
Endotoxins are frequent contaminants in plasmid DNA prepared from bacteria, and must be removed from the DNA to avoid unwanted inflammatory responses prior to in vivo applications such as gene therapy.
Test principle:
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ET. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ET.The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm.