Product name:Rat angiotensinogen,aGT ELISA Kit
Description:
Intended use
This immunoassay kit allows for the specific measurement of rat angiotensinogen, AGT concentrations in tissue homogenates, cell culture supernates, serum, and plasma and other relevant liquid.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for AGT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGT present is bound by the immobilized antibody. An biotinylated polyclonal antibody specific for AGT is added to the wells. Following a wash to remove any unbound reagent, an enzyme complex is added to the wells.After incubation and washing ,a substrate solution is added to the wells and color develops in proportion to the amount of AGT bound in the initial step. The color development is stopped and the intensity of the color is measured.