Product name:Tricine
CAS:5704-04-1
Molecular Formula:C6H13NO5
Formula Weight:179.17
Specification:25g
Description:
Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a lower negative charge than glycine allowing it to migrate faster. In addition its high ionic strength causes more ion movement and less protein movement. This allows for low molecular weight proteins to be separated in lower percent acrylamide gels. Tricine has been documented in the separation of proteins in the range of 1 to 100 kDa by electrophoresis.The tricine buffer at 25 mmol/L was found to be the most effective buffer among the ten tested for ATP assays using firefly luciferase.Tricine has also been found to be an effective scavenger of hydroxyl radicals in a study of radiation-induced membrane damage.