Product name:Tetramethylbenzidine
CAS:54827-17-7
Molecular Formula:C16H20N2
Formula Weight:240.34
Description:
Specification:100mg
Tetramethylbenzidine is a group with strong lipid solubility, so it is easy to enter the HRP reaction in cells and organelles. Moreover, due to the high lipid solubility, it is easy to form a polymer and produce thick and deep blue precipitate in the active part of HRP, which makes Tetramethylbenzidine become a good hair color group in the histochemical experiment. At the same time, the precipitation of the product can expose the active part of HRP and facilitate the enzymatic oxidation reaction.
The reaction product of Tetramethylbenzidine is dark blue, which is conducive to light microscopy, and the reaction product is more and more concentrated, often exceeding the range of single organelles (while DAB is limited to it). Therefore, the detection threshold of Tetramethylbenzidine reaction is relatively low.
Due to the above advantages, Tetramethylbenzidine is often used in the study of HRP and hrp-wga neural projection at the level of light microscope and ultrastructure. It should be noted that solution A and solution B in Tetramethylbenzidine should be prepared fresh within 2h. In addition, Tetramethylbenzidine is a strong skin irritant and has the potential to cause cancer. Therefore, gloves and ventilation should be used.