Product name:Isopropyl-beta-D-thiogalactopyranoside
CAS:367-93-1
Molecular Formula:C9H18O5S
Formula Weight:238.3
Specification:1g
Description:Isopropyl-beta-d-thiogalactopyranoside is a inducer with strong effects, which is not metabolized by bacteria and is very stable, so it is widely used by the laboratory. IPTG is often used in cloning experiments that require the induction of beta-galactosidase activity. It is often used in combination with x-gal or bluo-gal for blue-white screening of recombinant bacterial colonies that induce lac operon expression in escherichia coli. IPTG binds to lacI repressor protein and changes its conformation to play a role in preventing the inhibition of lacZ, the gene encoding beta-galactoside.
The isopropyl-beta-d-thiogalactopyranoside has the properties of inducing the synthesis of the enzymes, which are not broken down, and they are called consolation inducers. Because lactose induces the synthesis of the enzyme, but then breaks it down, causing many complex kinetic problems, people often use the inducer for various experiments.
Isopropyl-beta-d-thiogalactopyranoside is not metabolized by thalli, which is like lactose. Once they enter the cell, they have an induction effect that lasts for a long time. Therefore, the induction efficiency of IPTG is high. As the isopropyl-beta-d-thiogalactopyranoside is not metabolized, it has the specific induction of the expression of exogenous proteins inside the cell. It was not affected by cell metabolism and the induction effect was stable. Lactose has a dual effect, which can be the precursor of allactose as the inducer, and the carbon source, which is not stable during the induction process. The isopropyl-beta-d-thiogalactopyranoside is suitable for experimental research because of its excellent stability.
Melting point:105 ºC
Boiling point:438.4 ºC at 760 mmHg
Flash Point:219 ºC
Density:1.37 g/cm3
Chemical property:white crystalline powder
Product Categories:Substrates;13C & 2H Sugars;Biochemistry;Galactose;Glycosides;Sugars;
Thioglycosides;Enzyme substrates;Carbohydrates & Derivatives;Carbohydrates