Product name:Geneticin
CAS:108321-42-2
Molecular Formula:C20H40N4O10.2H2O4S
Formula Weight:692.71
Specification:100mg
Description:
G418 (also known as Geneticin (Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc)) is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is produced by Micromonospora rhodorangea. G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells. Resistance to G418 is conferred by the neo gene from Tn5 encoding an aminoglycoside 3‘-phosphotransferase, APH 3‘ II. G418 is commonly used in laboratory research to select genetically engineered cells (typically using the KanMX selectable marker). In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance.