Product name:DNA ligase
CAS:9015-85-4
Specification:200U
Description:
In molecular biology, DNA ligase is a special type of ligase, which is basically an enzyme (EC 6.5.1.1) that in the cell repairs single-stranded discontinuities in double stranded DNA molecules, in simple words strands that have double-strand break (a break in both complementary strands of DNA). Purified DNA ligase is used in gene cloning to join DNA molecules together. The alternative, a single-strand break, is fixed by a different type of DNA ligase using the complementary strand as a template[citation needed] but still requires DNA ligase to create the final phosphodiester bond to fully repair the DNA.
DNA ligase has applications in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in molecular biology laboratories for