Product name:Creatine phosphokinase
CAS:9001-15-4
Specification:1ku
Description:
Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase (and sometimes wrongly as creatinine kinase), is an enzyme (EC 2.7.3.2) expressed by various tissues and cell types. CK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). This CK enzyme reaction is reversible, such that also ATP can be generated from PCr and ADP.
Creatine phosphokinase is a white salt-free freeze-dried powder, soluble in water, with an optimal pH of 6.0-7.0(forward reaction) and 9.0(reverse reaction). Stability: deactivation under pH value less than 5, deactivation of enzyme by thawing and freezing; Under the condition of -20℃ and pH value of 6.6, stability can be maintained for 4 weeks in 0.1mot/L imidazole buffer, the stability is most stable in neutral 60% ethanol solution, and stability can be increased by adding 0.1% albumin or beta-mercaptoethanol in dilute solution. Freeze-dried products can be stored for 12 months at -20℃ and lose vitality for 6 months at 4℃. Inhibitors of mercury chloride acid, chelating agent, iodine acetic acid, melamine phosphate, adenosine, nitrite, sulfate, chloride, bromide, sulphite, fluoride thyroxine, 3, 5-2 nitro o-cresol, coenzyme I (NAD), L - three iodine formex glycine (T3), malonic acid salt, iodine, iodine (I -) acetic acid, orthophosphoric acid, pyrophosphate, melamine phosphate, such as activating agent have magnesium 2 +, Mn2 +, 2 - mercaptoethanol, dithiothreitol, glutathione, mercaptan n-acetyl cysteine, etc. The absorption coefficient at 280nm of 1% aqueous solution was 8.76. Enzyme reaction: creatine + adenosine triphosphate ═ adenosine diphosphate + creatine phosphate.
Creatine phosphokinase is an organ-specific enzyme found mainly in skeletal, cardiac and brain tissues. CPK catalyzes the synthesis of creatine (C) and adenosine triphosphate (ATP) from phosphocreatine (CP) and adenosine diphosphate (ADP) in muscles.