Asia Bio-Pharmaceutical Research Institute

Agarose

Product name:Agarose
CAS:9012-36-6
Molecular Formula:C10H15N3O3
Specification:5g
Description:
Agarose is the neutral component of AGAR without charge. The chemical structure of agaran is composed of long chains linked alternately by 1, 3-linked beta-d-galactose and 1, 4-linked 3, 6-ether-l-galactose [1-2].

Polysaccharides derived from red algae are mainly composed of polygalactose, of which about 70% are agarose and 30% are branched agarose. Agarose has a linear structure, consisting of d-galactose and 3, 6-dehydrated galactose alternately joined by beta 1,4 and alpha 1,3 to form duplicate disaccharide units. The agarose branched off from the beta 1,3 bond. Substances extracted from seaweed using hot water contain about 40% AGAR. Agar-agar, often in a sheet or loose rope form, is often used as food colloid, pharmaceutical packaging, or bacterial culture media. Pure agarose is often used in biochemical laboratories as a semi-solid support for electrophoresis, chromatography and other technologies for the separation and analysis of biological macromolecules or small molecules.

The separation of nucleic acids by agarose gel electrophoresis is mainly based on their relative molecular weight and molecular configuration, and is also closely related to the concentration of gel.

 

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