Metallothionein's detection method is mainly to calculate the concentration of Metallothionein by protein content determination. Immunology is the most important method. It is especially suitable for micro-detection, has sensitive and specific characteristics, sensitivity is pg/ml level, and has good detection effect on Metallothionein which has little content in body fluid. In recent years, Western Blotting, Radioimmunoassay (RIA) and Enzyme Linked Immunosorbent Assay (ELISA) have been established.
The RIA method was first established by Mallie et al. in 1979 when the content of Metallothionein in rat serum was determined. Mulder et al. then established an RIA for determining the MT content in human body fluid. Zahir Shaidh A et al reported the use of RIA to detect urine in cadmium-contaminated areas. Liquid Metallothionein content. The detection range of this method is between 0.1 and 100 ng/ml, but this method requires a radioisotope, which takes about 3 days, which is inconvenient for detection.
In 1986, Thomas et al. established an ELISA method that was not significantly different from the RIA method. The sensitivity was up to 100 pg/ml. Compared with RIA, it was relatively fast and did not require expensive equipment and exposure to radioisotopes. In 1995, Akintola et al. established an ELISA for the determination of Metallothionein in human plasma and urine using MT-1 to prepare antibodies with a detection limit of 5 ng/ml.
Metallothionein can be detected by protein analysis
