Indole-3-acetic acid mainly comes from the reaction of indoles and acetic acid. In 3L stainless steel autoclave, add 270g (4.1mol) 85% potassium hydroxide, 351g (3mol) indoles, and then slowly add 360g (3.3mol) 70% glycolic acid solution. Heat airtight to 250℃ and stir for 18h. Cool to below 50℃, add 500ml water, stir at 100℃ for 30min to dissolve indoles 3-potassium acetate. Cooling to 25℃, the autoclave material into water, add water to the total volume of 3L. Indole -3- acetic acid precipitation was precipitated by 500ml ether extraction and hydrochloric acid acidification at 20-30℃. Filter, wash with cold water and dry under light, and get the inole-3-acetic acid455-490g.
Indole-3-acetic acid is used as the plant growth hormone and analytical reagent. Several species of linin such as 3-indoleacetic acid, 3-indoleacetaldehyde, 3-indoleacetonitrile and ascorbic acid exist naturally in nature. Tryptophan is the precursor of the biosynthesis of 3-indoleacetic acid in plants. The basic function of lintalin is to regulate plant growth, not only to promote growth, but also to inhibit growth and organ building. As well as existing in a free state in plant cells, it can also be used to bind with biomolecules and other binding forms, as well as to form bonds with special substances.