1, fluorescence method
Fluorescence method is to use the fluorescence characteristics of porphyrin in hemin. The fluorescence intensity of the sample can be detected by fluorophotometer to calculate the corresponding heme content, such as the quantitative determination of hemoglobin in feces, and the digestion of hemoglobin in the intestine. For heme, hemoglobin is mostly digested into porphyrin under the action of bacteria in the intestine, and the corresponding heme content can be calculated by using the fluorescence characteristics of porphyrin. The heme and the oxalic acid reagent are combined to remove the ferrous ion and are reduced to a porphyrin having a fluorescent property. Under this condition, the porphyrin measured by the fluorescence method is the total amount of hemoglobin in the intestinal tract. Digested hemoglobin divided by the total amount of hemoglobin is the hemoglobin intestinal conversion rate, because the higher the bleeding site, the longer the digestion and absorption, the higher the intestinal conversion rate, so the conversion rate from the intestinal tract can be approximated Estimate the location of intestinal bleeding.
2. Visible spectrophotometry
The hemin was dissolved in a 0.25% Na2CO3 solution and had a maximum absorption peak at a wavelength of 610 nm. The heme content was determined by the method in the hydrochloric acid acetone extraction process of hemin.
3, polarographic method
By studying the polarographic behavior of hemin, 0.1mol/L NH3-NHCl was used as a buffer solution, and a hemoglobin showed a sensitive cathodic wave at -0.42V. Using this method, the minimum hemin concentration can be detected to be 8 x 10 mol/L.
4, electrode method
The method uses the modified graphite electrode to detect hemin. It is found that the electrode reacts sensitively to the concentration of hemin. The concentration of heme in the range of 1×10～1×10mol/L is linear with the electrode reaction.
Method for detecting hemin
